We urge the community to sequence their “e2141“-bearing lab strains prior to future publication or dissemination. We note that in the prior literature, strains designated as “e2141” may have been carrying the e2144 (c2785t) mutation. In light of our sequencing results, the literature indicates that the e2144 (c2785t mutation variously attributed to “e2141” or “e2144“) results in a much stronger embryonic phenotype than e2141 (c2920t a3610g mutations). See, for example, Mello et al., 1994, and Hutter and Schnabel, 1994. The e2141 allele is in the CGC collection as strain CB4037, and the e2144 allele is now deposited at the CGC as strain GE68.
Hutter H and Schnabel R. (1994). glp-1 and inductions establishing embryonic axes in C. elegans. Development 120, 2051-64.
Kodoyianni V, Maine EM, and Kimble J. (1992). Molecular basis of loss-of-function mutations in the glp-1 gene of Caenorhabditis elegans. Mol. Biol. Cell 3, 1199-213.
Mello CC, Draper BW, and Priess JR. (1994). The maternal genes apx-1 and glp-1 and establishment of dorsal-ventral polarity in the early C. elegans embryo. Cell 77, 95-106.
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