We have recently published an analysis of deficiencies affecting recombination of LGV Left using array Comparative Genomic Hybridization (Jones et al., 2007). A beneficial outcome of this research is the creation of a deficiency map anchored precisely to the physical genome. With breakpoints positioned to within 4kb in most cases the deleted gene complement within each deficiency is known. Such a map can be used to reassess old mapping data and compile current mapping with the confidence that gene candidate lists are definitive. We have included a table of the regions containing the physical breakpoints in the in the deficiencies shown below.

Breakpoint regions for deficiencies on LGV Left.

  Left Breakpoint Region (bp) Right Breakpoint Region (bp)
Strain Name Outside Inside First Deleted Gene Inside Outside Last Deleted Gene
BC3955 sDf53 NA 0 B0348.5 304585 305898 F33E11.3
BC3461 sDf74 NA 0 B0348.5 393345 393346 nhr-254
BC1381 sDf38 NA 0 B0348.5 436506 437592 B0554.2
BC3463 sDf39 NA 0 B0348.5 667033 667034 nhr-180
BC1784 sDf72 NA 0 B0348.5 1019751 1020703 R09A1.2
BC1785 sDf32 NA 0 B0348.5 1235161 1259870 trim-9
BC3470 sDf33 NA 0 B0348.5 1715178 1717073 srw-100
BC3473 sDf52 NA 0 B0348.5 1805691 1807257 hsp-16.41
BC1999 sDf40 NA 0 B0348.5 3250138 3254512 T28A11.12
BC1554 sDf45 NA 0 B0348.5 3250138 3254512 T28A11.12
BC1782 sDf34 393563 393631 nhr-254 3336275 3336484 C17B7.5
BC1289 sDf28 1485433 1494558 C38C3.10 4221738 4236163 nlp-9
BC3539 sDf50 2053451 2057412 Y40B10A.2 4454741 4471106 gnrr-5
BC1230 sDf27 2740536 2741462 srg-62 4636769 4642667 F54D11.3
BC3436 sDf44 5784865 5785487 F44E7.7 5939394 5942577 M03F8.7
BC3957 sDf36 5886349 5895968 grd-9 6318973 6319012 T28C12.4
BC3472 nDf18 6404431 6404470 F57F4.4 6576477 6576616 fut-2
BC3541 sDf30 5578210 5579194 klc-2 7527936 7530080 C54F6.6
BC3217 sDp30 5580489 5580535 klc-2 7527936 7530080 C54F6.6
BC2511 sDf29 8378016 8378729 F38E1.11 9958684 9958802 T07C12.9
BC3462 sDf35 7918271 7928375 srsx-5 10515304 10515679 C35A5.7