Worm Breeder's Gazette 9(3): 68

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

A Consensus Sequence 5' of C. elegans Core Histone Genes

S. Roberts, S. Emmons and G. Childs

Figure 1

Comparison of DNA sequences surrounding six C. 
tone genes reveals a novel conserved sequence 
located approximately 80 nucleotides upstream of the translational 
start site. The six genes include all of the genes in one genomic 
cluster (one of each of the four core histone genes), and two from 
another, unlinked cluster (one H2A gene and one H4 gene). All of the 
conserved sequences 5' of each of these genes are presented in the 
figure below. Starting at the ATG codon and moving upstream, they 
include a sequence adjacent to the translational initiation site 
typically found in eukaryotic mRNAs, a conserved sequence surrounding 
the transcriptional initiation site similar to one found in some sea 
urchin histone genes, a TATA box, and the novel sequence. With the 
exception of yeast, upstream consensus sequences in histone genes of 
other organisms are restricted to the genes for individual classes of 
histone proteins (i.e., they are H4 gene-specific or H2A gene-specific,
etc.). In yeast a conserved sequence of 16 nucleotides activates 
transcription of histone genes in a cell cycle-dependent manner (Osley,
M. H., Gould, J., Kim, 5., Kane, M. and Hereford, L. (1986) Cell 46, 
537-544). Like the C. , this 16-mer is found 
upstream of different classes of histone-encoding genes.
In the figure, numbering of nucleotides begins at the first 
translated nucleotide, and the number of non-matching nucleotides 
between each conserved region is given. The asterisk indicates the 
site of transcriptional initiation in two of the genes (+/- 1 
nucleotide) determined from Sl mapping experiments. The arrow over the 
consensus transcriptional initiation sequence indicates the direction 
of transcription.

Figure 1