Worm Breeder's Gazette 9(3): 59
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
DNAfragments from -HK2 clone which contained paramyosin gene were processed and get results as follows. Nucleotide sequences of the HindIII 5kb were and HindIII 1.4kb fragments were nearly completed. There many ORFs with predicted-helix protein structure. Polarity of ORF was determined from the amino acid sequence predicted with the nucleotide sequence. Many fusion proteins(Miller et al, Pro. N. A. S.(1986) 83, 2305-2309) were isolated and were characterized by immunoassay. One of them which was corresponded to the 3'end part of the HindIII 5kb fragment crossreacted with a monoclonal antibody which only crossreacted with full sized paramyosin. Our results and the information from Stephan D. Rioux(personal communication and the worm gazette, Vol. 9, No.2, p39), Tc1 insertion site of TR605 munant located at the 3' region of the gene. There were three polyA site in the HindIII 1.4 kb fragment. Although we are not sure which site is a real polyA site the result suggests that Tc1 insertion into 3' noncording region might decrease the termination of the message. C. a fashionable organism to study in Japan. I do hope you could have a time to attend a workshop in Japan near future.