Worm Breeder's Gazette 9(3): 56

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

C. elegans cDNA Libraries

J. Ahringer, D. Lawson, T. Rosenquist and J. Kimble

We have constructed two  -gt10 cDNA libraries from RNA isolated from 
a mating population of C. luded 70% 
hermaphrodites and 30% males among the adults. The average insert gize 
is 1200 bp with 3.0 x 105 independent clones for one library and 1600 
bp with 8 x 106 independent clones for the other. Characterization of 
the libaries is in initial stages: screening 200,000 plaques of the 
former library with genomic fem-3 DNA as a probe, we isolated 8 
positive plaques. Screening 200,000 plaques of the latter library with 
the same piece of fem-3 DNA, we found 2 positive clones.
We are also starting to make stage specific cDNA libraries using RNA 
isolated from eggs, 12 hr, 24 hr, 36 hr, 48 hr, and 60 hr (25 ) 
animals. The eggs and staged animals include both hermaphrodites and 
males. Once these libraries are made, they will be available to anyone 
for use.