Worm Breeder's Gazette 9(3): 38

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Continuation of the Saturation Screen for EMS Induced Recessive Lethals on LGV (Left)

R.C. Johnsen and D.L. Baillie

We are continuing our attempt to saturate and map essential genes 
and steriles on the left half of Linkage Group V (see Johnsen and 
Baillie WBG Vol.9 #2).
We have screened 10,900 'chromosomes', treated with 0.012M EMS. We 
have isolated 751 lethal and sterile mutations: 242 strictly on LGV, 
451 strictly on LGIII and 29 lines with a lethal on both LGV and LGIII.
All lines but the ones carrying mutations strictly on LGV have been 
We have begun the task of complementation testing our 242 mutations 
against the deficiencies that divide LGV(left) into zones (see 
Rosenbluth et. al. WBG Vol.9 #2). For sDf30, 34 out of 179 mutations 
tested fail to complement, indicating 19% of our lethals lie in zones 
11 and 12. For sDf27, 9 out of 64 mutations fail to complement giving 
14% of our hits in zones 7,8 and 9.
The research has been supported by NSERC and MDA(Canada) grants to