Worm Breeder's Gazette 9(3): 21

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Genetic and Molecular Characterization of mec-14

N. Hom, J. Way and M. Chalfie

We have isolated seven EMS-induced mec-14 mutants in a screen for 
touch insensitive animals. The gene maps between sma-3 and lin-13 on L.
G. III. None of the alleles is an amber mutation, but there is no 
enhancement of the mutant phenotype (touch insensitivity) in 
heterozygotes of the canonical mutation (u55) and a deficiency for the 
gene (nDf16). We have not seen any abnormalities in the touch cells (
ALM, AVM, or PVM) in electron micrographs the mutants.
We also isolated a spontaneous mec-14 mutant from TR679. The mutant 
contains a 4.6 kb EcoRI fragment that cosegregates with the mec-14 
mutation. We have cloned this (minus the Tc1 which was excised using 
Ba1I) into pUC18, and have used the resulting plasmid (pTU4) to probe 
genomic DNA from wild type and mutant strains. N2 contains a single 
hybridizing band at 3.0 kb; a spontaneous revertant and its Mec 
sibling yielded bands of 3.0 and 4.6 kb, respectively. One of the EMS-
induced alleles (u82) had an unusual EcoRI fragment (it was slightly 
smaller). All together these data suggest that we have cloned, at 
least, part of the mec-14 gene.
John Sulston and Alan Coulson have located the mec-14 gene (using a 
lambda clone) to the middle of a fairly large contig (approx. 200 kb). 
Since a number of genes lie within 0.1 map units of mec-14 (between 
the ends of nDf16 and nDf20 - lin-16, lin-13, 
be a very useful contig. We are 
going to see if we can find the endpoints of the deficiencies and, if 
the contig extends far enough, the eT1 breakpoint on III.