Worm Breeder's Gazette 9(2): 79
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
We have been analyzing a set of 30 1500 r gamma-induced lethal mutations using the sDp2 lethal recovery system. sDp2 is a free duplication of the left third of LGI which we have been using as a 'balancer' for lethals in the region it covers. The recovered lethals have the genotype sDP2/ (let-x unc-13/ dpy-5) the wild-type allele of dpy-5 but not unc-13, all lethal mutations that were recovered have an Unc-13 phenotype. The wild-type alleles carried by sDp2 will not recombine with the normal homologues. We have been mapping these lethals with respect to dpy-5 and unc-13 and complementing them with visibles and EMS-induced lethals. Of the 30, 13 are to the left and 8 to the right of dpy-5 Of the 13 to the left, 9 have been complementation tested; one of these may be a deletion and another, h330, is a viable allele of unc-11 recovered as a synthetic lethal with dpy-5 8 to the right, one h168, fails to complement several loci including let-78 and has been designated hDf2. Of the remaining 9 mutations, one, h53 fails to complement two EMS- induced mutations to the right of dpy-5 and is not as yet separable from dpy-5. It is possible that other mutations which did not give recombinants with dpy-5 are also deletions, perhaps even taking out dpy-5. We are complementation testing these now. One of the strains which gave no recombinants appears to be carrying an X;I translocation. It was detected initially by a high frequency of males from the self-cross progeny of a heterozygote. There is evidence of pseudolinkage between lon-2 (X) and unc-87 (I). Other markers on the X are being tested. We have mapped 51 EMS-induced lethals, 30 to the left of dpy-5 and 21 to the right. They define 21 complementation groups to the left and about 18 to the right. A note to all gene mappers out there -- If you are working with anything on LGI that might be under sDp2, please make a double of it with dpy-5. We have found that it is a lot easier to do complementation tests if everything is linked to a common marker. We will be happy to test anything against our deletions if you send it to us or the Stock Center as a dpy-5 double mutant.