Worm Breeder's Gazette 9(1): 77

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

More About Dosage Compensation

B. Meyer and L. Casson

In response to appreciated criticism at the Cold Spring Harbor worm 
meeting, we have extended our biochemical analysis of dosage 
compensation to control for possible complications caused by 
differences in anatomy and hence gene expression in males and 
hermaphrodites.  We have measured the relative transcript levels of 
the two myosin genes (myo-1(I),  expressed in the 
pharynx, a tissue which is nearly identical in males and 
hermaphrodites.  (We made gene-specific probes from clones of myo-1 
and myo-2 obtained from J.  Karn.) Transcript levels for myo-2 were 
found to be identical in X0 males and XX hermaphrodites when 
normalized to the levels of myo-1.  In addition the level of myo-2 in 
hermaphrodites bearing a mutation in either dpy-21, 
ted by 2-3 fold compared to the 
level of myo-2 in wild-type hermaphrodites (when normalized to myo-1).  
This set of observations is in agreement with those we presented at 
the meeting using act-1, omal probes and 
other X-specific probes.
In our assays act-4(X) behaves differently from other X-specific 
genes in that the level of act-4 is not elevated in dpy-21, 
odites although the level in 
males and hermaphrodites appears to be identical (when normalized to