Worm Breeder's Gazette 8(3): 83

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Molecular Cloning of lin-12

I. Greenwald

I have examined a total of eight independent lin-12 null mutations 
that arose spontaneously in a predominantly Bergerac genetic 
background.  I probed genomic Southern blots with a 1.2 kb wild-type 
HindIII fragment that was defined by an insertion mutation (see last 
Newsletter) and/or with a cosmid 'contig' obtained from Alan Coulson 
and John Sulston that extends approximately 20 kb in each direction 
from the 1.2 kb HindIII fragment.  The results thus far indicate that (
1) seven of the eight mutations are associated with Tc1 insertions (2) 
the insertions are at different sites, and (3) all the insertion sites 
may lie within a single Pst fragment that is 2.8 kb in wild-type.