Worm Breeder's Gazette 8(3): 79

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Tc1 Polymorphisms in the ced-3 Region of LGIV

H. Ellis, R. Horvitz

Figure 1

Wild-type function of ced-3 is required for the initiation of 
essentially all programmed cell deaths during C.  elegans development. 
In the absence of ced-3 function, cells that would normally die 
instead survive and differentiate.  These results suggest that ced-3 
might be involved in the determination of the fates of certain cells (
in this case, in the decision between death and survival).  We hope to 
analyze the function of ced-3 at a molecular level.  As a preliminary 
approach to the cloning of ced-3 we have mapped Bristol-Bergerac Tc1 
polymorphisms in the region around ced-3 IV (see figure below).  To 
identify Tc1 polymorphisms that map near ced-3 we constructed 
Bristol/Bergerac hybrid strains that carried a small portion of 
Bergerac DNA around the ced-3 locus and Bristol DNA elsewhere.  These 
strains were then used in 3-factor crosses with marked Bristol strains 
to genetically map these polymorphisms.  We found that a 4.9 kb EcoR1 
Tc1-containing band in Bristol is tightly linked to ced-3 (p<0.1%).  A 
5.1 kb (EcoRI) Bristol-specific Tc1 band and an approximately 2.7 kb (
EcoRI) Bergerac-specific Tc1 band map between ced-3 and unc-26 (which 
are about 0.2% apart) and have not been separated from each other 
genetically.  We have identified two cosmids containing the 4.9 kb 
Bristol-specific Tc1 band and plan to use these cosmids to begin 
walking in this region.
[See Figure 1]

Figure 1