Worm Breeder's Gazette 8(1): 34

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RFDs as Probes for Cloning

A. Rose, D. Baillie, K. Beckenbach, D. Nelson, N. Mawji

Figure 1

We have been screening for restriction fragment differences (RFDs) 
between the N2 and Bo strains as described by Emmons, et al., 1979.  
Random N2 fragments with EcoRI and HindIII ends were cloned into 
pBR322, isolated, and purified.  Using these fragments as P nick 
translated probes, RFDs were detected about one per six tested probes. 
We are in the process now of accumulating and mapping a set of these 
probes.  It is our hope to establish a reasonably large collection of 
mapped RFD probes that might be utilized in the cloning of genes with 
no biochemically identified products.  Towards this end we have mapped 
four such 
One of these pGe s18 has been further mapped to the left of unc-13 (
I).  Terry Snutch has screened our Charon 4 library for corresponding 
lambda clones of this region, which we have restriction mapped and 
subcloned for chromosome walking.  We hope that characterization of 
the DNA adjacent to pCe s18 will allow us to isolate some of the genes 
in the unc-15 region described by Rose and Baillie, 1980.
Interestingly, probe pCe s18 when hybridized against N2, Bo, and FF 
shows the N2 banding pattern.  We would welcome information on the 
relationship (if any) between Bo and FF.
[See Figure 1]

Figure 1