Worm Breeder's Gazette 8(1): 34
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
We have been screening for restriction fragment differences (RFDs) between the N2 and Bo strains as described by Emmons, et al., 1979. Random N2 fragments with EcoRI and HindIII ends were cloned into pBR322, isolated, and purified. Using these fragments as P nick translated probes, RFDs were detected about one per six tested probes. We are in the process now of accumulating and mapping a set of these probes. It is our hope to establish a reasonably large collection of mapped RFD probes that might be utilized in the cloning of genes with no biochemically identified products. Towards this end we have mapped four such RFDs: One of these pGe s18 has been further mapped to the left of unc-13 ( I). Terry Snutch has screened our Charon 4 library for corresponding lambda clones of this region, which we have restriction mapped and subcloned for chromosome walking. We hope that characterization of the DNA adjacent to pCe s18 will allow us to isolate some of the genes in the unc-15 region described by Rose and Baillie, 1980. Interestingly, probe pCe s18 when hybridized against N2, Bo, and FF shows the N2 banding pattern. We would welcome information on the relationship (if any) between Bo and FF. [See Figure 1]