Worm Breeder's Gazette 8(1): 23

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

More Monoclonal Antibodies to Germline Granules

Y. Tabuse, J. Miwa

Figure 1

In a previous newsletter (WBG 7[1]:75), we described the making of a 
library of monoclonal antibodies (MoAbs) to C.  elegans in order to 
identify tissue- or cell-specific antigens in the animal.  We recently 
reported on three germline-specific MoAbs, two of which react with 
granules unique to germline cells (Yamaguchi et al.  1983.  Develop., 
Growth and Differ.  25:121-131).
In an attempt to obtain more germline-specific (and also early 
embryo-specific) MoAbs, we used fertilized eggs and 1- to 4-cell stage 
embryos for immunization.  Of 800 hybridomas obtained by fusing P3U-1 
myeloma cells to splenocytes from immunized BALB/c mice, about 60 
hybridomas were found to secrete antibodies to C.  elegans by indirect 
immunofluorescent staining of C.  elegans whole mount preparations.  
Five hybridoma lines, 704D, 904A, 911F, 1003B, and 1108E, out of these 
60 were identified as secretors of Abs that react with germline 
granules (G-granules).  We are at present characterizing these cell 
lines together with the line M32 isolated previously.
The table shows preliminary results on the cross-reactivity of the 
Abs to various nematodes.  The Abs of all 6 hybridomas reacted with 
the G-granules of both C.  elegans and C.  briggsae, possibly 
reflecting the taxonomic closeness.  However, the Ab of only one 
hybridoma line, 1003B, stained G-granules of a free-living diecious 
nematode recently isolated from soil of Wakayama-ken.  The nematode 
Bursaphelenchus is parasitic and considered a prime culprit of recent 
disastrous damage to pine trees in Japan.  The G-granules of this 
nematode were stained also with the 1003B Ab and tentatively with 904A 
and M32.
The result with the 1003B Ab indicates that the G-granules of all 
the nematodes listed here possess some structure common to them.  The 
difference observed of cross-reactivity among the Abs may reflect 
either various antigenic determinants of a single element in the 
granules or a complex of multiple elements constituting the granules.  
Further study on cross-reactivity and biochemical characterization are 
under way.
[See Figure 1]

Figure 1