Worm Breeder's Gazette 7(2): 59
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
In the genetic tests performed thus far, all seven mutants behave virtually identically. All are (at least semi-) dominant and closely linked to dpy-13 locus on the linkage group IV. There is no evidence as yet for any maternal effects in the expression of amanitin resistance or sensitivity, Heterozygous (ama(R)/+) males were mated with dpy-8 odites to test X linkage or dominance. The mated hermaphrodites were cloned in microtiter wells in amanitin, The sensitive, self progeny (DpyUnc) did not grow beyond the L1 stage, but ama(R) F1 hermaphrodites with normal movement and body shape developed to maturity along with the DpyUnc males. The results were the same for each of the seven mutants. The production of resistant (ama(R)/+) hermaphrodite progeny shows that ama(R) is dominant. The production of resistant males shows that it is autosomal. Crosses with strains carrying two linked chromosomal markers showed that the ama(R) allele, m118 is not linked to standard reference markers on LG I, II or III and V, but it is closely linked to dpy-13(IV). (The chromosomal markers were the following: dpy-3( DR39)unc-6(e78)(x); 21)unc-3(e151)(x); ) unc-75(e950)(I); )unc-54(e1301)(I); unc-4(II); unc-4(II); daf-2(e1370)unc-32(III); daf-7 I); unc-24(IV); unc-24(IV); unc-22(IV); ; dpy11 unc-51(V).) The preliminary two- factor crosses produced only one dpy-13 ama(R)/dpy-13 + recombinant among 100 homozygous Dumpy segregants. This suggests that ama(m118) locus may be within one map unit of dpy-13. Subsequent crosses revealed that all the seven ama(R) alleles are closely linked to dpy- 13. Three-factor crosses with the m118 in trans to recessive markers, +ama(R)+ unc-22, produced three of the four possible classes of recombinants: Unc-Resistant, tive, and Dpy- Sensitive DpyUnc progeny were invariably Sensitive (40/40 tested). In the total of six recombinants, two were obtained in the interval between dpy-13 and ama(R), and four recombinants were obtained in the interval between ama(R) and unc-22. Obviously, these data are preliminary, however, since all the ama(R) mutants are dominant and all appear to be closely linked to dpy-13(IV) we suspect that they all may be allelic.