Worm Breeder's Gazette 7(1): 94
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
We reported previously that in the presence of 1 g/ml tumor promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) or phorbol-12,13-didecanoate (PDD) C. elegans neither grows nor reproduces and develops severely uncoordinated movement (WBG 5:52) and that the three TPA-resistent mutants thus far studied all belong to the same complementation group and all are linked to chromosome IV ( CSH abstracts, 1981). We have analyzed eight more TPA-resistent mutants in detail. In the presence of 1 g/ml TPA, all of the 11 mutants move normally, reproduce well with a brood size (BRS) of about 200 at 20 C, and propagate. The mutation defined by each of these mutants is semidominant to the wild-type allele in that heterozygotes are able to reproduce with a BRS of about 40 at 20 C in 1 g/ml TPA. The complementation analysis for these mutants was based on the brood size and movement of the F1 cross progeny between the reference mutant MJ500 and the other 10 mutants. Those F1 cross progeny exhibited essentially the brood size and movement of the homozygous parents. The 11 mutants are all assigned to linkage group IV by two-factor crosses. In three-factor crosses using the double marker mutant dpy-9 of 370 Dpy non-Unc F2 progeny segregated TPA- resistent ants. These results indicate that we have yet found only one gene (we name it tpa-1) involved in TPA action on C. elegans and that the tpa-1 locus maps close to dpy-9 on linkage group IV. We are doing a finer mapping of tpa-1. Any suggestions concerning markers for mapping or anything else would be appreciated.