Worm Breeder's Gazette 7(1): 94

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

More on Mutants Resistant to Tumor Promoting Phorbol Esters

Y. Tabuse, J. Miwa

We reported previously that in the presence of 1 g/ml tumor 
promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) or 
phorbol-12,13-didecanoate (PDD) C.  elegans neither grows nor 
reproduces and develops severely uncoordinated movement (WBG 5[2]:52) 
and that the three TPA-resistent mutants thus far studied all belong 
to the same complementation group and all are linked to chromosome IV (
CSH abstracts, 1981).  We have analyzed eight more TPA-resistent 
mutants in detail.
In the presence of 1 g/ml TPA, all of the 11 mutants move normally, 
reproduce well with a brood size (BRS) of about 200 at 20 C, and 
propagate.  The mutation defined by each of these mutants is 
semidominant to the wild-type allele in that heterozygotes are able to 
reproduce with a BRS of about 40 at 20 C in 1 g/ml TPA.  The 
complementation analysis for these mutants was based on the brood size 
and movement of the F1 cross progeny between the reference mutant 
MJ500 and the other 10 mutants.  Those F1 cross progeny exhibited 
essentially the brood size and movement of the homozygous parents.  
The 11 mutants are all assigned to linkage group IV by two-factor 
crosses.  In three-factor crosses using the double marker mutant dpy-9 
of 370 Dpy non-Unc F2 progeny segregated TPA-
resistent ants.
These results indicate that we have yet found only one gene (we name 
it tpa-1) involved in TPA action on C.  elegans and that the tpa-1 
locus maps close to dpy-9 on linkage group IV.  We are doing a finer 
mapping of tpa-1.  Any suggestions concerning markers for mapping or 
anything else would be appreciated.