Worm Breeder's Gazette 7(1): 85

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Gottingen Worm Group activities 1981 and Prospects 1982

After Gunter von Ehrenstein's death of a heart attack December 26, 
1980, the 6 directors of the Max-Planck-Institute for Experimental 
Medicine decided to close the Department of Molecular Biology as of 
the end of 1982.  We are continuing work to finish up and publish work 
in progress with gradual attrition as people find new positions 
elsewhere and with correspondingly diminishing budget.  A list of our 
recent papers and manuscripts is given below.
Eddi Isnenghi completed his paper on the phenotypes of the Gottingen 
emb mutants (including TSP's and maternal tests) and is trying to 
microinject cloned DNA to rescue worm mutants.  He is also trying to 
transform yeast mutants with worm DNA.  Randy Cassada is still trying 
to map embs on LG III with deficiencies and duplications and is 
looking for new strains of C.  elegans in the wild.  Eddi has received 
a 6-months' extension of his DFG (= NSF) fellowship until July.  Ken 
Denich is writing up his work on emb mutant lineages in Gottingen for 
a paper and as PhD Thesis for the Department of Zoology, University of 
Manitoba and will be returning there this summer to finish up PhD 
requirements.  Einhard Schierenberg has continued with laser-induced 
cell fusion experiments.  If the membrane between 2 cells is disrupted 
at the right time, the division of both nuclei results in 4 more or 
less normal cells.  Development continues to a twitching lima bean 
monster.  Fusing cells in later stages results in uncoordinated 
animals.  All treated embryos show timing defects in the E-cell line 
similar to emb mutants, perhaps due to remixing of cytoplasm.  A copy 
of Einhard's film on embryogenesis is available (with commentary) from 
Scott Emmons for those who want to show it to students.  Einhard is 
going to Bill Wood's lab this fall.  Khosro Radnia has left for a job 
in industry.  Ulli Certa and Franz Scharfenberg are characterizing 
histone H2A variants in emb mutants.  They have also identified 
several histone H4 DNA clones in a C.  elegans genomic library using a 
sea urchin probe obtained from Mike Grunstein.  Ulli has finished his 
PhD and plans to go to Grunstein's lab at UCLA this spring for a post-
doc on yeast histones.  Ulli and the other gene cloners have been 
working successfully with similar minded members of the Institute's 
Department of Chemistry.  Franz hopes to do his PhD in that department.
Shahid Siddiqui has just joined Joe Culotti at Northwestern.  Tom 
Cole is very happy with his new Zeiss EM 109 electron microscope.  Our 
computer-aided reconstruction program with color display now works for 
LM and EM input.  We are trying to transfer the programs and data to 
the MRC, so they will still be available after our department has 
closed.  Chris Carlson has gone to the EMBL in Heidelberg as of Jan 1 
to work on computer graphics of molecules.  Ursula Reuter also has a 
position at EMBL as of Jan 1 as an EM technician.  Ed Hedgecock 
visited for 3 weeks this fall to show us microinjection, antibody 
staining (with anti-actin from Mary Osborne and Klaus Weber), post-
embryonic lineage techniques and the like.  It was a very useful and 
pleasant visit.  We hope to have some of you as visitors in