Worm Breeder's Gazette 7(1): 75

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Anti - C. elegans Monoclonal Antibodies

Y. Yamaguchi, M. Furusawa, J. Miwa

Figure 1

To identify tissue- or cell-specific antigens in C.  elegans, we are 
isolating hybridoma cell lines that produce antibodies to C.  elegans.  
About 300 hybridoma cell lines were obtained by fusing P3U1 myeloma 
cells to spleen cells from BALB/c mice immunized with homogenates of 
fertilized C.  elegans eggs or of whole larvae and adult animals.  By 
indirect immunofluorescent staining of frozen sections of a mixed 
population covering hatchees to gravid adults, the cell lines were 
screened for secretion of antibodies to C.  elegans tissues and 
checked for distribution of antigens in animals.  We have so far 
examined some eighty monoclonal antibodies that react with eggs, 
larvae, and adults in a reproducible manner.
The monoclonal antibodies thus tested are classified as 
[See Figure 1]
We are investigating the more detailed spacial distribution patterns 
of antigens as recognized by monoclonal antibodies and the time of 
their appearance in tissues and cells during embryonic and post-
embryonic development.

Figure 1