Worm Breeder's Gazette 7(1): 59

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Analysis of Three Messages By Cytological Hybridization

M.K. Edwards

Using the technique of cytological hybridization described here a 
year ago, I have analyzed RNA's complementary to three cloned DNA's 
from C.  elegans genomic libraries: collagen, actin, and myosin.
The collagen probe hybridizes specifically to the dorsal and ventral 
hypodermal cells in the adult.  Collagen messages cannot be detected 
in the ovary or in early embryos.  They are observed in later embryos 
beginning at 2 to 3 hours after the 2-cell stage at 25 C; their time 
of appearance corresponds to that of poly-A-containing message as 
determined by Hecht et al.  (Devel.  Biol.  83:374, 1981).
Due to the conservation of actin coding sequences, the actin probe 
hybridizes to transcripts for muscle and cytoplasmic actins.  
Cytological hybridizations show that actin messages are abundant in 
the ovary and are stored in the egg as maternal RNAs.  Additional 
expression occurs during embryogenesis and in adult body-wall muscle, 
pharynx, and spermatheca.
In contrast with the actin probe, the myosin clone hybridizes 
primarily to transcripts of the myosin gene expressed in body-wall 
muscle.  There is little cross-hybridization to other myosin 
transcripts.  The most intense hybridization occurs in muscles, while 
little hybridization is seen in the intestine or ovary.  In addition, 
the myosin probe does not hybridize to early embryos, but does 
hybridize strongly to older embryos undergoing morphogenesis and