Worm Breeder's Gazette 7(1): 57
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
Using a combination of 2D gels and Northern blot analysis we have been able to demonstrate that the regulation of the spermatogenic pathway in the hermaphrodite occurs at the level of transcription at least with respect to the major sperm protein 15K. 2D gel analysis of pulse-labeled synchronous cultures of N2 shows that 15K is not synthesized in L1 or L2 larvae but is synthesized in L4 larvae during spermatogenesis and is then repressed after sperm production has stopped in gravid hermaphrodites. Northern blot analysis using our cloned genomic probe for 15K mRNA has demonstrated the lack of 15K mRNA in L2 and gravid hermaphrodites and its presence only during spermatogenesis in the hermaphrodite.