Worm Breeder's Gazette 6(1): 19
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
UV in the proper dose (60 W; 22 cm; 15'') seems to be a useful tool for inducing somatic recombination in C. elegans. Embryos of dpy-5 ( e61) + + unc-54 (e1301) /+ unc-15 (e1214) + + and also of dpy-5 (e61) + + unc-54 (e1301) / + + unc-13 (e1091) + genotypes were irradiated, collected for 2h and grown at 15 C. Non-dumpy, partially uncoordinated hermaphrodites were scored. The plates were then transferred to a 25 C environment for 8h. Non-dumpy, partially- paralyzed hermaphrodites and dumpy non-paralyzed males were scored. All putative mosaics were studied under a Zeiss Universal polarized light microscope. The total fraction of real mosaics was about 3%, the majority of them being unc-54 mosaic (partially paralyzed only at high temperature). There were also unc-15 and dumpy male mosaics, together with animals having no detectable muscle defect. This project, discussed with O. Siddiqi et al., has been carried out in Szeged. [See Figure 1]