Worm Breeder's Gazette 6(1): 19

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Some Parameters of Mitotic Recombination Induced by UV in C. elegans

A. Fodor

Figure 1

UV in the proper dose (60 W; 22 cm; 15'') seems to be a useful tool 
for inducing somatic recombination in C.  elegans.  Embryos of dpy-5 (
e61) + + unc-54 (e1301) /+ unc-15 (e1214) + + and also of dpy-5 (e61) 
+ + unc-54 (e1301) / + + unc-13 (e1091) + genotypes were irradiated, 
collected for 2h and grown at 15 C.  Non-dumpy, partially 
uncoordinated hermaphrodites were scored.  The plates were then 
transferred to a 25 C environment for 8h.  Non-dumpy, partially-
paralyzed hermaphrodites and dumpy non-paralyzed males were scored.  
All putative mosaics were studied under a Zeiss Universal polarized 
light microscope.  The total fraction of real mosaics was about 3%, 
the majority of them being unc-54 mosaic (partially paralyzed only at 
high temperature).  There were also unc-15 and dumpy male mosaics, 
together with animals having no detectable muscle defect.  This 
project, discussed with O.  Siddiqi et al., has been carried out in 
[See Figure 1]

Figure 1