Worm Breeder's Gazette 5(1): 9
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
The isolation and first characterization about a balancer chromosome temporarily called Bal-X-1 was presented at the Cold Spring Harbor meeting. This chromosome when heterozygous with an X chromosome marked by dpy-8 and unc-3 suppress crossover on this region. Characterizing the Bal-X-1 further on, we found, that Bal-X-1 viable only in heterozygous or hemizygous form. Bal-X-1 has been marked with lon-2(e678) allele. Bal-X-1 (lon) males are perfectly normal and fertile. Hermaphrodites heterozygous for Bal-X-1 always segregate males about 8-20% frequencies. The males are XO. Bal-X animals homozygous for her-1(e1520) (kindly provided by Jonathan Hodgin) are viable but almost completely sterile. Even if they lay some eggs, their oldest progeny has never been larger than L1, which die soon. Bal-X-1 can balance the following mutants: mn-110 (BH); let-2(e1470) (A.F.); let-15(e1471) (A.F.). We are going on to learn, whether Bal-X-1 is a translocation. In these experiments, we use stocks of double homozygous for two markers ( one in the medium and one in one of the ends the autosome) of the different autosomes and measure the recombination between them. If Bal-X-1 is a translocation like Dp-1 (BH) we expect, that the recombination frequency will be changed on the autosome being involved.