Worm Breeder's Gazette 5(1): 7a
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
Our collection of lethal and sterile mutations near unc-3 X and balanced by mnDp1 now numbers 51--9 steriles and 42 lethals. These define 24 genes, with eight genes having more than one allele; five of these eight have three or more alleles, including let-1 with six and let-2 with 16. We are continuing to map the mutants, using 20 deficiencies of the region and five duplications of unc-3.We are also testing the lethals and steriles with the informational suppressor sup- 5. Testing for dominant suppression of a lethal is done by crossing sup-5/+ males to mnDp1/+; unc-3 let, and looking for Unc progeny ( males if they survive to adulthood) older than the normal lethal phase. Dominant suppression of a rescuable sterile is tested by mating sup- 5/+ males to unc-3 he let is a rescuable sterile) and looking again for Unc progeny. Testing recessive suppression is more complicated and takes advantage of the difference between sup-5 4) homozygotes (wild type) and sup-5 dpy-18 heterozygotes (Dpy-ish), which allows us to pick homozygous sup-5 from the sup-5 dpy-18 heterozygote. The lethal stage of sup-5 unc-3 red with the nonsuppressed unc-3 let. [Neither unc-3(e151) nor unc-7(e139), markers used in the procedure, is suppressed by sup-5.]So far, eight of 29 mutants are suppressed. One mutant weakly suppressed in sup-5/+ has not been tested yet in sup-5 homozygotes. Three lethals are suppressed only in sup-5 homozygotes; one lethal is suppressed better in sup-5 homozygotes than in sup-5/+, and two lethals are weakly suppressed in both sup-5/+ and sup-5. One lethal suppressed only in sup-5 homozygotes and let-5(mn106), a maternal effect lethal that is strongly suppressed in sup-5/+ and sup-5, have been maintained as fertile sup-5; let stocks.