Worm Breeder's Gazette 5(1): 7a

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Title unknown.

Authors unknown.

Our collection of lethal and sterile mutations near unc-3 X and 
balanced by mnDp1 now numbers 51--9 steriles and 42 lethals.  These 
define 24 genes, with eight genes having more than one allele; five of 
these eight have three or more alleles, including let-1 with six and 
let-2 with 16.  We are continuing to map the mutants, using 20 
deficiencies of the region and five duplications of unc-3.We are also 
testing the lethals and steriles with the informational suppressor sup-
5.  Testing for dominant suppression of a lethal is done by crossing 
sup-5/+ males to mnDp1/+; unc-3 let, and looking for Unc progeny (
males if they survive to adulthood) older than the normal lethal phase.
Dominant suppression of a rescuable sterile is tested by mating sup-
5/+ males to unc-3 he let is a rescuable 
sterile) and looking again for Unc progeny.  Testing recessive 
suppression is more complicated and takes advantage of the difference 
between sup-5 4) homozygotes (wild type) and sup-5 
dpy-18 heterozygotes (Dpy-ish), which allows us to 
pick homozygous sup-5 from the sup-5 
dpy-18 heterozygote.  The lethal stage of sup-5 
unc-3 red with the 
nonsuppressed unc-3 let.  [Neither unc-3(e151) nor unc-7(e139), 
markers used in the procedure, is suppressed by sup-5.]So far, eight 
of 29 mutants are suppressed.  One mutant weakly suppressed in sup-5/+ 
has not been tested yet in sup-5 homozygotes.  Three lethals are 
suppressed only in sup-5 homozygotes; one lethal is suppressed better 
in sup-5 homozygotes than in sup-5/+, and two lethals are weakly 
suppressed in both sup-5/+ and sup-5.  One lethal suppressed only in 
sup-5 homozygotes and let-5(mn106), a maternal effect lethal that is 
strongly suppressed in sup-5/+ and sup-5, have been maintained as 
fertile sup-5; let stocks.