Worm Breeder's Gazette 5(1): 32b

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

A Temperature-sensitive Morphology Abnormal Mutant (JK 64)

R. Hosono

A temperature-dependent morphologically abnormal mutant was isolated,
which develops into an adult with dumpy phenotype at 15 C but with 
roller phenotype at 25 C in addition to dumpy.  At 25 C, the dumpy 
phenotype appeared within L1 stage and was complete at L3 stage and 
the roller phenotype was also appeared within L3 stage.  The 
heterozygous offspring( F1 ), produced by crossing the mutant 
hermaphrodite with wild-type males, were rollers.  Therefore, the 
dumpy is autosomal recessive and the roller is dominant in 
heterozygote but temperature-sensitive in homozygote.  The F2 progeny 
produced by self-fertilization of the F1 roller hermaphrodite 
segregated to wild, dumpy, and roller animals in a ratio of 1:1:2.  
The dumpy mutation in the mutant was shown to be an allele of dpy-10 ( 
LG II ).  To segregate the roller mutation from the dumpy, 103 F2 
roller individuals were cloned, but no homozygous roller progeny was 
obtained.  Therefore, the dumpy and the roller phenotypes are produced 
by closely linked mutations or perhaps by a single mutation.  In the 
mutant, the epistatic effect of the dumpy to roller may be temperature-
sensitive.