Worm Breeder's Gazette 3(2): 21b
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
We have examined the early cleavages of our ts zyg mutants by Nomarski optics and measured the number of nuclei in the embryos as a function of time at restrictive temperature using Hoechst stain. We find that all of the strict maternal mutants (M,M) either have abnormal early cleavages or never progress beyond 200 nuclei or both. Two of the other zyg mutants display abnormal early cleavages. Interestingly these are the paternal mutant B235 (M,N,P) and the anomalous mutant B261 (M,N,?). None of the other mutants displays abnormal early cleavages or stops before 200 nuclei. The first cleavages can be schematically diagrammed as follows for N2 and for some of the mutants: [See Figure 1]