Worm Breeder's Gazette 3(2): 19b
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
Mutations causing transformation of XO animals (normally male) into fertile hermaphrodites have been isolated by the following means. A strain of genotype him-5(e1490) 28)V; X was constructed: this strain segregates 70% Dpy odites and 30% non-Dpy unc males, because the him-5 marker causes high self progeny male frequency and the dpy-21 marker (hitherto incorrectly assigned to LGX) is not expressed in XO males. The unc-7 marker was included to avoid outcrossing. After mutagenesis of this strain, non-Dpy Unc hermaphrodites were picked from the F2 generation, and some carried mutations causing transformation of XO animals into fertile hermaphrodites. So far, three independent isolates have been found to map to LGV, and have been assigned to a new locus, her-1V maphroditization'). Preliminary results indicate that her homozygotes are fertile hermaphrodites regardless of X chromosome dosage, and that mutations in all three tra genes are epistatic to her-