Worm Breeder's Gazette 2(2): 5b
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
We study the embryonic cell lineage on cleaving eggs using Nomarski optics and recordings on video tape. Eggs are observed outside the parent. Care was taken that air bubbles are trapped under the microscope slide. Under these conditions at an air temperature of 20 C-22 C animals hatch 13-14 hours after fertilization. We have determined the lineage of 182 cells: 128 AB-, 16 MST-, 16 C-, 16 E-, 4 D- and 2 p4-descendents. Cell divisions generally are synchronous within each lineage. Each lineage has its autonomous rhythm of cell division. This rhythm is maintained in spite of extensive cellular rearrangement. The length of the cell cycle depends on the point of origin of the stem cells in the cytoplasm of the egg. The most anterior AB-lineage has the shortest and the posterior P-lineage the longest cell cycle. Early events of embryogenesis like blastula- and gastruIa formation will be described.