Worm Breeder's Gazette 2(2): 5a

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Title unknown.

Authors unknown.

A method for fixing and embedding C.  elegans eggs will be 
introduced.  It involves prolonged osmium or glutaraldehyde-osmium 
fixation at elevated temperatures.  This procedure works on all stages 
of embryogenesis.  16 embryos in various stages ranging from an 
uncleaved zygote to a prehatching 'pretzel' have been serially 
sectioned.
The analysis of a 5-hour embryo with 294 cells and of a 7-hour 
embryo with 540 cells will be presented.  Data from the 5-hour embryo 
supplement the Nomarski results.  Data from the 7-hour embryo will be 
compared with the anatomy of the young L1 worked out by Sulston and 
Horvitz.