Worm Breeder's Gazette 2(2): 24a
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
Several of the more interesting ts zygote defective (zyg) mutants isolated by Hirsh and Vanderslice (1) map in the unc4-dpy1O region of linkage group II. We have begun isolation of additional lethal mutations in this region by the following procedure: Heterozygous dpy10(e128) +/+ unc4(e120) worms are mutagenized and F1's of wild phenotype are cloned at 25 C. Clones that fail to segregate one of the two morphological markers are scored as carrying a nonmaternal lethal linked to that marker. Unc and dpy worms from clones that segregate both are tested for sterility; if one of the two marker phenotypes is found to be sterile the clone is scored as carrying a maternal lethal linked to that marker. Lethals are maintained as heterozygotes by passing phenotypically normal worms. From a first screening of 1,004 F1 clones following mutagenesis, five maternal and four nonmaternal lethals were isolated. Two of the maternal mutants are zyg, two are gonadogenesis defective, and one is spermatogenesis defective (sp). One of the zyg lesions and the sp lesion are temperature sensitive. All the nonmaternal mutants, are blocked during larval development; none of them are temperature sensitive. All nine mutants have been mapped roughly relative to dpy10 and unc4 by scoring segregation of the lethal from the morphological marker it is linked to. The two zyg mutants map between e128 and e120, but they complement each other,and both complement the zyg mutant tsB244 (2), which maps in the same region.