Worm Breeder's Gazette 2(2): 23b
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
We are identifying recessive lethal mutations in the region of unc-3 of the X chromosome, using DP(X;V)1 as a balancer. To date, 21 such mutants have been found following EMS mutagenesis. Sixteen of these have been fully complemented and belong to eleven genes; repeats have been found in three genes. Several mutants have been mapped and define genes on each side of unc-3 and unc-7. Both complementation and mapping are continuing. We estimate that there are 25 genes in the region covered by the duplication. We have so far only roughly described the phenotypes of the mutants. Five of the 21 seem to be embryonic lethals; four of these five fall into one complementation group, although certain pairs of these mutants show interallelic complementation. Eleven mutants are arrested at various larval stages. One mutant appears sex-specific in that hermaphrodites die as juveniles but males develop to adults. One mutant shows a strong maternal effect: the homozygous mutant progeny of a heterozygote are fertile, but their progeny die before adulthood. The other mutants are sterile adults. One of these appears to make defective sperm.