Worm Breeder's Gazette 2(2): 19

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Isolation of a Non Temperature-sensitive Incomplete Sex Transformer Mutant, TRI, in C. elegans Var. Bergerac

B. Beguet

The reproduction of hermaphrodite C.  elegans has been studied using 
mas or fes mutations affecting spermatogenesis or oogenesis.  The 
different genotypes show important reproductive changes from the wild-
type.  Also, mutations controlling sexual dimorphism can affect the 
worms reproduction.
An incomplete sex transformer mutant tri has been isolated.  This 
recessive mutation tri transforms one part of an XX hermaphrodite's 
progeny into phenotypic males, the other part having hermaphroditic 
phenotype is also partially transformed because the hermaphrodite 
exhibits a male tail with a vestigial copulatory bursa.  These 
incompletely transformed hermaphrodites can be sterile or produce few 
progeny : 13 progeny at 18 C instead of 140 progeny for wild-type var. 
Bergerac.  The fecund worms have a hermaphroditic gonad with both 
sperm and oocytes.  No chromosomal anomalies during spermatogenesis or 
oogenesis can be observed with light microscopy.  However worms that 
have produced progeny show enlargement's anomalies for ovocytes after 
the gonad's loop.  It suggests that this mutation could also disturb 
enlarging oocytes.  This non conditional mutant can be compared for 
its sexual phenotypic expression with tsB202 mutant studied by KLASS 
et al.  (1976).