Worm Breeder's Gazette 17(4): e1 (May 1, 2003)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
| 1 | Institute of Internal Medicine, 2a, Vladimirovskii spusk, Novosibirsk, 630003, Russia |
| 2 | bakaev@online.nsk.su |
The purpose of this study was to investigate the effect of acetylsalicylic acid in water solutions on the nematode life span. In this experiment acetylsalicylic acid was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without acetylsalicylic acid) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without acetylsalicylic acid in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with acetylsalicylic acid in any concentration. This investigation was carried out in temperature +21°C and in the darkness. The obtained results are presented in the following table.
| Concentration of acetylsalicylic acid (mg/L) | n | Longevity (days) | |
| Mean±S.E. | Maximal | ||
| Control | 36 | 9.14±0.50 | 15 |
| 100 | 36 | 11.36±0.78 | 21 |
| 10 | 36 | 11.78±0.77 | 21 |
| 1 | 36 | 13.14±0.74 | 21 |
Conclusion: If acetylsalicylic acid solution was applied to C. elegans in concentration of 100; 10 and 1 mg/L, it was able to increase significantly (P>0.01; >0.02 and >0.001) their mean longevity in comparison with control to 24.29; 28.88 and 43.76 percent.
Acknowledgment: The authors wish to express their thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.