Worm Breeder's Gazette 17(4): 24 (May 1, 2003)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
| 1 | Institute of Internal Medicine, 2a, Vladimirovskii spusk, Novosibirsk, 630003, Russia |
| 2 | bakaev@online.nsk.su |
The purpose of this study was to investigate the effect of hydrogen peroxide in water solutions on the nematode life span. In this experiment hydrogen peroxide was used in following dilutions: 100 mg/L, 10 mg/L, 1 mg/L, 0.1 mg/L, 0.01 mg/L and 0.001 mg/L. Three adult animals (3-5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without hydrogen peroxide) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without hydrogen peroxide in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with hydrogen peroxide in any concentration. This investigation was carried out in temperature +21°C and in the darkness. The obtained results are presented in the following table.
| Concentration of hydrogen peroxide | n | Longevity (days) | |
| Mean±S.E. | Maximal | ||
| Control | 12 | 12.42±1.57 | 18 |
| 100 mg/L | 12 | 11.92±1.72 | 20 |
| 10 mg/L | 12 | 10.33±1.39 | 17 |
| 1 mg/L | 12 | 9.17±1.48 | 20 |
| 0.1 mg/L | 12 | 7.42±0.87 | 16 |
| 0.01 mg/L | 12 | 8.92±1.34 | 19 |
| 0.001 mg/L | 12 | 10.33±1.45 | 19 |
Conclusion: If hydrogen peroxide solution was applied to C. elegans, it was not able to increase their mean longevity significantly in comparison with control.
Acknowledgment: The authors wish to express their thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.