Worm Breeder's Gazette 17(4): 23 (May 1, 2003)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
| 1 | Institute of Internal Medicine, 2a, Vladimirovskii spusk, Novosibirsk, 630003, Russia |
| 2 | bakaev@online.nsk.su |
The purpose of this study was to investigate the effect of glycerol in water solutions on the nematode life span. In this experiment glycerol was used in following dilutions: 100 g/L, 10 g/L, 1 g/L, 100 mg/L, 10 mg/L, 1 mg/L and 0.1 mg/L. Three adult animals (3-5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without glycerol) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without glycerol in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with glycerol in any concentration. This investigation was carried out in temperature +21°C and in the darkness. The obtained results are presented in the following table.
| Concentration of glycerol | n | Longevity (days) | |
| Mean±S.E. | Maximal | ||
| Control | 12 | 18.08±1.60 | 26 |
| 100 g/L | 12 | 16.00±1.93 | 21 |
| 10 g/L | 12 | 16.08±1.44 | 24 |
| 1 g/L | 12 | 15.67±1.85 | 27 |
| 100 mg/L | 12 | 16.83±1.66 | 26 |
| 10 mg/L | 12 | 16.00±2.12 | 26 |
| 1 mg/L | 12 | 10.75±1.47 | 21 |
| 0.1 mg/L | 12 | 13.00±1.56 | 24 |
Conclusion: If glycerol solution was applied to C. elegans, it was not able to increase their mean longevity significantly in comparison with control.
Acknowledgment: The authors wish to express their thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.