Worm Breeder's Gazette 17(1): 65 (October 1, 2001)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
| 1 | Box 45, Novosibirsk, 630107, Russia |
| 2 | E-mail: bakaev@online.nsk.su |
The purpose of this study was to investigate the effect of different concentrations of vitamin B12 in water solutions on nematode life span. In this experiment vitamin B12 was used in following dilutions: 1:104, 1:105, 1:106, 1:107, 1:108 and 1:109. Three adult animals (3 5 days old) were kept in microtitre wells containing 0,5 ml of liquid medium (with E. coliand without vitamin B12) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with vitamin B12 in any concentration) every day (one worm in one well) beginning from third day. This investigation was carried out in temperature +210C and in the darkness.
The obtained results are presented in the following table.
| Concentration of vitamin B12 | n | Longevity (days) | ||
| Mean±S.E. | Maximal | |||
| Control | 12 | 17,8±1,9 | 30 | |
| 1:104 | 12 | 18,8±2,3 | 32 | |
| 1:105 | 12 | 19,7±1,6 | 37 | |
| 1:106 | 12 | 15,3±1,1 | 26 | |
| 1:107 | 12 | 13,9±1,0 | 28 | |
| 1:108 | 12 | 17,4±1,1 | 30 | |
| 1:109 | 12 | 16,6±1,2 | 30 | |
Conclusion: If vitamin B12 solution was applied to C. elegans, it was not able to increase significantly their mean longevity in comparison with control.