Worm Breeder's Gazette 17(1): 48 (October 1, 2001)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Towards a Pristionchus genome map- I P. pacificus BAC Library construction and end sequencing of BAC clones

Ralf J Sommer1, Taco Jesse2, Stephan C. Schuster3, Günther Raddatz3, Heike Keller3, Christa Lanz3

1 Dept of Evolutionary Biology, Max Planck Institute for Developmental Biology, Tuebingen, Germany
2 KeyGene NV , Wageningen, The Netherlands
3 Genome Centre, Max Planck Institute for Developmental Biology, Tuebingen, Germany

In order to facilitate the easier cloning of genes in the satellite model system Pristionchus pacificus, we have initiated a genomic approach by constructing genetic and physical maps of Pristionchus pacificus. An essential prerequisite for a good genomics approach is the availability of a good genomic library with large insert sizes.

We constructed a BAC library of Pristionchus pacificus. The library contained 13, 440 clones in total and was a result of 4 different ligations. The average insert size of the clones is 128 kb. Insert sizes ranged between 100-180kb. We estimate the genome coverage of the library to be approximately 8 times. End sequencing of the BAC clones was done at the Genome Centre of the MPI Tübingen. Uptil now 6000 BAC clones have been end sequenced. On an average approximately 600 bp were read from each BAC end. The sequence was checked for quality using the PHRED/PHREP protocol. The total length of the BAC ends sequenced was approximately 10 Mb. The GC content of the sequence was 47%. From the 11271 ends sequenced, 8232 BAC ends gave no hits and 1129 gave hits to the non-redundant NCBI database.