Worm Breeder's Gazette 16(5): 46 (February 1, 2001)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

An attempt  to slow aging in C. elegans. 19. A positive effect of 1-butylbiguanide hydrochloride

Vladimir V. Bakaev

Box 45, Novosibirsk, 630107, Russia E-mail: bakaev@online.nsk.su

The purpose of this study was to investigate the effect of different concentrations of 1-butylbiguanide hydrochloride (this medicine is widely used for treatment of patients with diabetes mellitus) in water  solutions on nematode life span.  In this experiment 1-butylbiguanide hydrochloride was used  in  following dilutions: 1:103,1:104,1:105,1:106,1:107 and 1:108. Three adult animals (3 - 5 days old) were kept in microtitre wells containing  0,5  ml of liquid  medium  (with E. coli and without 1-butylbiguanide hydrochloride) during 4 hours,  then they  were  discarded  and newborn larvae were transferred in next wells (with 1-butylbiguanide hydrochloride in any concentration) every day  (one  worm in one well) beginning from third day.  This investigation was carried out in temperature +210C and in  the  darkness.

The obtained results are presented in the following table.

Concentration of 1-butylbiguanide hydrochloride

n

Longevity (days)

Mean±S.E.

Maximal

Control

12

 14,25±1,30

23

1:103

12

 13,08±0,70

22

1:104

12

 17,58±1,01

29

1:105

12

 15,75±0,86

24

1:106

12

 15,58±0,91

23

1:107

12

 15,33±0,89

24

1:108

12

 14,00±0,62

23

 

Conclusion: If 1-butylbiguanide hydrochloride solution was applied to C. elegans,  it was  able to increase their mean (by 23,37%, p<0,05) as well as maximal longevity in comparison with control in dilution of 1:104.

Acknowledgment: The author  wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.