Worm Breeder's Gazette 16(5): 46 (February 1, 2001)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
Box 45, Novosibirsk, 630107, Russia E-mail: bakaev@online.nsk.su
The purpose of this study was to investigate the effect of different concentrations of 1-butylbiguanide hydrochloride (this medicine is widely used for treatment of patients with diabetes mellitus) in water solutions on nematode life span. In this experiment 1-butylbiguanide hydrochloride was used in following dilutions: 1:103,1:104,1:105,1:106,1:107 and 1:108. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,5 ml of liquid medium (with E. coli and without 1-butylbiguanide hydrochloride) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with 1-butylbiguanide hydrochloride in any concentration) every day (one worm in one well) beginning from third day. This investigation was carried out in temperature +210C and in the darkness.
The obtained results are presented in the following table.
Concentration of 1-butylbiguanide hydrochloride | n | Longevity (days) | |
Mean±S.E. | Maximal | ||
Control | 12 | 14,25±1,30 | 23 |
1:103 | 12 | 13,08±0,70 | 22 |
1:104 | 12 | 17,58±1,01 | 29 |
1:105 | 12 | 15,75±0,86 | 24 |
1:106 | 12 | 15,58±0,91 | 23 |
1:107 | 12 | 15,33±0,89 | 24 |
1:108 | 12 | 14,00±0,62 | 23 |
Conclusion: If 1-butylbiguanide hydrochloride solution was applied to C. elegans, it was able to increase their mean (by 23,37%, p<0,05) as well as maximal longevity in comparison with control in dilution of 1:104.
Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.