Worm Breeder's Gazette 16(5): 45 (February 1, 2001)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

An attempt to slow aging in C. elegans. 18. No effect of sodium fusidine

Vladimir V. Bakaev

Box 45, Novosibirsk, 630107, Russia E-mail: bakaev@online.nsk.su

The purpose of this study was to investigate the effect of different concentrations of  sodium fusidine  in water solutions  on nematode life span.  In this experiment sodium fusidine was  used  in following  dilutions: 1:103,1:104,1:105,1:106,1:107 and 1:108. Three adult animals (3 - 5 days old) were kept in microtitre wells containing  0,75  ml of liquid  medium  (with E. coli and without sodium fusidine) during 4 hours, then they  were  discarded and newborn larvae were transferred in next wells (with sodium fusidine in any concentration) every day  (one  worm in one well) beginning from third day.  This investigation was carried out in temperature +210C and in  the  darkness. The obtained results are presented in the following table.

Concentration of sodium fusidine

n

Longevity (days)

Mean±S.E.

Maximal

Control

36

 9,47±0,76

21

1:103

36

10,03±0,81

23

1:104

36

 9,44±0,88

25

1:105

36

 8,72±0,51

18

1:106

36

 9,86±0,94

26

1:107

36

 8,86±0,73

24

1:108

36

 8,86±0,73

15

 

 

Conclusion: If sodium fusidine solution was applied to C. elegans,  it  was not able to increase their mean as well as maximal longevity in comparison with control.

Acknowledgment: The  author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.