Worm Breeder's Gazette 16(3): 33 (June 1, 2000)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

An attempt to slow aging in C. elegans. 16. no effect of kanamycin

Vladimir V. Bakaev1,2

1 Box 45, Novosibirsk, 630107, Russia
2 E-mail: bakaev@online.nsk.su

The purpose of this study was to investigate the effect of different concentrations of kanamycin sulphate in water solutions on nematode life span. In this experiment kanamycin sulphate was used in following dilutions: 1:101, 1:102, 1:103, 1:104, 1:105, 1:106 and 1:107. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and without kanamycin sulphate) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with kanamycin sulphate in any concentration) every day (one worm in one well) beginning from third day. This investigation was carried out in temperature +210C and in the darkness.

The obtained results are presented in the following table.

 

Concentration of kanamycin sulphate

n

Longevity (days)

Mean S.E.

maximal

Control

12

14,75 1,44

22

1:101

12

7,17 0,42

9

1:102

12

7,17 0,34

9

1:103

12

12,33 2,10

25

1:104

12

13,67 1,13

21

1:105

12

12,42 1,62

22

1:106

12

14,75 1,58

23

1:107

12

12,58 1,27

21

 

Conclusion: If kanamycin sulphate solution was applied to C. elegans, it was not able to increase their mean as well as maximal longevity in comparison with control.

Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.