Worm Breeder's Gazette 16(3): 13 (June 1, 2000)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Transposon-tagged genes and strains available.

H. Laloux, E. Martin, L. Segalat

CNRS-CGMC, University of Lyon, 69100 Villeurbanne, France

As an alternative to the systematic gene-directed knock-out program, we
are exploring ways to create collections of transposon-tagged genes. Our
strategy is to generate strains containing identified transposons
insertions. Rubin and collaborators of the Drosophila gene disruption
project are currently following a similar strategy with the fly genome
(Genetics vol 153, p135). Over the last couple of years, we have run
several pilot experiments making use of natural worm transposons to
assay the feasibility of the strategy. In practice, we are following an
approach which has been pioneered in the Plasterk lab. Starting from
mut-7 or similar mutator backgrounds, we propagate the strain over a few
generations to generate clones. Then, we determine the position of new
insertions of Tc1, Tc3 and Tc5 by a modification of the transposon
insertion display protocol described in the WBG (Vol 14, n!4 page 20),
in which DNA flanking transposons can be amplified by anchored PCR, and

Over 500 clones have been generated in the course of the pilot
experiments. In addition to insertions generated in our lab, a few
clones have been contributed by Satis Sookhareea and Michel Labouesse. A
total of 400 different insertions of Tc1, Tc3 and Tc5 have been
identified (most clones we generate contain 0 to 2 insertions). The
distribution of the insertions on the genetic map does not reveal any
major bias. Over 50% of insertions located in annotated genome regions
fall in genes. Introns tend to slightly favor insertions because of
their high TA content, which is a target for the transposons we are

The strains we produce are available to the C. elegans community upon
Please consult our site at
http://cgmc.univ-lyon1.fr/transposon%20project.htm for an updated list
of lines and hits.
Requests should be addressed to Laurent Segalat