Worm Breeder's Gazette 16(2): 49
Box 45, Novosibirsk, 630107, Russia. E-mail: firstname.lastname@example.org
The purpose of this study was to investigate the effect of different concentrations of dicaine hydrochloride in water solutions on nematode life span. In this experiment dicaine hydrochloride was used in the following dilutions: 1:104, 1:105, 1:106 and 1:107. Three adult animals (3-5 days old) were kept in microtitre wells containing 0.75 ml of liquid medium (with E. coli and without dicaine hydrochloride) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with dicaine hydrochloride in any concentration) every day (one worm in one well) beginning from the third day. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table.
Concentration of dicaine hydrochloride n Longevity (days) mean +/- S.E. Maximal Control 12 12.8 +/- 1.7 28 1:104 12 18.2 +/- 2.2 30 1:105 12 14.2 +/- 1.5 24 1:106 12 16.0 +/- 1.9 25 1:107 12 14.9 +/- 2.0 26
Conclusions: If dicaine hydrochloride was applied to C. elegans, it was able to increase their mean as well as maximal longevity only in concentration 1:104 (P<0.05) in comparison with control.
Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.