Worm Breeder's Gazette 16(1): 60 (October 1, 1999)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
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The purpose of this study was to investigate the effect of different concentrations of procaine hydrochloride in water solutions on nematode life span. In this experiment procaine hydrochloride was used in following dilutions: 1:103, 1:104, 1:105 and 1:106. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.75 ml of liquid medium (with E. coli and without procaine hydrochloride) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with procaine hydrochloride in any concentration) every day (one worm in one well) beginning from third day. This investigation was carried out in temperature +21°C and in the darkness. The obtained results are presented in the following table.
Concentration of procaine hydrochloride
9.7 + 0.6
11.3 + 0.8
11.3 + 1.0
14.3 + 1.9
11.2 + 0.9
Conclusion: If procaine hydrochloride solution was applied to C. elegans, it was able to increase their mean longevity [by 47.4 per cent, statistically significant (P < 0.05)] in dilution 1:105.
Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.