Worm Breeder's Gazette 15(4): 46 (October 1, 1998)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
Box 45, Novosibirsk, 630107 Russia
C. elegans larvae (1-2 days old) were collected from separate stock, frozen and after 12 hours or more time, thawed, centrifuged and mixed with S medium containing E. coli (1:1), this mixture was used as a medium for worms in experimental group. The medium for the control group was prepared by mixing S medium containing E. coli with S medium without E. coli (1:1). Three adult animals (3-5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and with or without larvae extract) during 12 hours, then they were transferred in next wells everyday (one worm in one well). Dilutions of larvae extract (1:10, 1:100 and 1:1000) were applied on third day. This investigation was carried out in temperature +24C and in the darkness. The obtained results are presented in the following table. Dilutions of Longevity (days) larvae extract n mean +/- S.E. maximal No dilution 12 toxic 1:10 12 19.9 +/- 1.5 25 1:100 12 15.0 +/- 1.4 27 1:1000 12 14.6 +/- 1.5 22 Control 12 16.0 +/- 1.6 20 Conclusion: If the larvae extract was applied to C. elegans, it was able slightly to increase their mean longevity (by 24.4 percent, not significant) in dilution 1:10. Probably extract prepared from larvae C. elegans contains any factor, which can slightly stimulate longevity of adult worms, but this effect is obviously insufficient for noticeable prolonging of longevity. Acknowledgment: The authors wish to express his thanks to CGC for providing C. elegans(Bristol, N2) and E. coli OP50.