Worm Breeder's Gazette 15(4): 13 (October 1, 1998)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
The Sanger Centre, Wellcome Trust Genome Campus, Cambridge, UK and the Genome Sequencing Center, Washington University, St. Louis, MO, USA.
Over 85 megabases of C. elegans genomic DNA has now been finished, whilst over 98% of the physical map sequence is either finished or represented by preliminary contig assemblies. The breakdown for the C. elegans chromosomes is as follows:- I=11.9 II=13.6 III=10.3 IV=13.1 V=18.6 X=17.4 In the available sequence data 18,394 protein coding genes have been predicted of which 159 currently have been shown to possess splice variants. 40% of the predicted genes are transcriptionally confirmed from EST data. Seven of the eleven telomeric clones isolated by Wicky et al (Proc.Nat.Acad.Sci.USA (1996) 93:8983-8988) have either direct sequence linkage to the sequence map or confirmed linkage through PCR products of 4.5 to 11kb. These are LGIR:cTel29B; LGIIL:cTel52S; LGIIIL:cTel54X; LGIVL:cTel4X; LGIVR:cTel79B; LGVL:cTel3X; LGXR:cTel55X. Any corrections to gene predictions or any information which will improve the annotation for sequence data is greatly appreciated and should be directed to Steve Jones (firstname.lastname@example.org) or John Spieth (email@example.com). All finished sequences are available after completion and unfinished sequences are available after completion of the initial shotgun phase. Sequences are available by anonymous ftp and the WWW. The latter offers on-line search facilities:- Sanger Center, UK ftp: ftp.sanger.ac.uk directory: pub/databases/C.elegans_sequences WWW: http://www.sanger.ac.uk/Projects/C_elegans Genome Sequencing Center ftp: genome.wustl.edu directory: pub/gsc1/sequence/st.louis/elegans/ WWW: http://genome.wustl.edu/gsc/ The entire set of predicted proteins, including those from alternatively spliced transcripts, are available as the database WormPep, see http://www.sanger.ac.uk/Projects/C_elegans/wormpep. Preliminary sequences for entire chromosomes are now available. These sequences are available via http://www.sanger.ac.uk/Projects/C_elegans/chromosomes.shtml. Data from the C. elegans genome project is available as an ACEDB database. For information regarding the C. elegans ACEDB database contact Richard Durbin(firstname.lastname@example.org) or see http://www.sanger.ac.uk/Projects/C_elegans/acedb.shtml. ACEDB data releases can be retrieved via anonymous ftp from:- USA: ncbi.nlm.nih.gov in repository/acedb UK: ftp.sanger.ac.uk in pub/acedb France: lirmm.lirmm.fr in genome/acedb A version of the C. elegans acedb database is now available on-line. This database is updated weekly. See http://www.sanger.ac.uk/Projects/C_elegans/webace_front_end.shtml. This weekly updated database can also be accessed using the xaceclient, see http://www.sanger.ac.uk/Projects/C_elegans/xaceclient_howto.shtml.