Worm Breeder's Gazette 15(2): 57 (February 1, 1998)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
| 1 | P.O. Box 45, Novosibirsk, 630107, Russia |
| 2 | N.N. Petrov Research Institute of Oncology, St. Petersburg, 189646, Russia |
There are data on the life span prolonging effect of pineal peptide preparation epithalamin in mice, rats and Drosophila melanogaster [1,2]. The purpose of this study was to investigate the effect of different concentrations of epithalamin in water solutions on nematode life span. In this experiment epithalamin was used in following dilutions : 1:10*5, 1:10*6, 1:10*7, 1:10*8, 1:lO*9 and 1:10*10. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.75 ml of liquid medium (with E. coli and without epithalamin) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with epitalamin in any concentration) every day (one worm in one well). This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Table I Concentration of Longevity (days) epithalamin n mean +/- S.E. maximal Control 12 17.4 +/- 2.5 29 1:10*5 12 12.8 +/- 2.1 27 1:10*6 12 15.1 +/- 1.9 28 1:10*7 12 17.1 +/- 2.2 28 1:10*8 12 19.6 +/- 2.2 29 1:10*9 12 18.8 +/- 2.0 31 1:10*10 12 18.7 +/- 1.7 25 Table II Concentration of Longevity (days) epithalamin n mean +/- S.E. maximal Control 12 23.5 +/- 2.3 33 1:10*7.5 12 16.8 +/- 2.5 34 1:10*8.0 12 19.3 +/- 1.7 33 1:10*9.0 12 13.7 +/- 1.4 21 Conclusion: If the water solution of epithalamin was applied to C. elegans during the whole life span in above described conditions, it was not able to prolong the life span of these animals. 1. Anisimov V.N., Khavinson V.Kh., Morozov V.G. Twenty years of study on effects of pineal peptide preparation: Epithalamin in experimental gerontology and oncology. Ann.N.Y.Acad. Sci., 1994, 719: 483-493. 2. Anisimov V.N., Mylnikov S.V., Oparina T.I., Khavinson V Kh. Effect of melatonin and pineal peptide preparation epithalamin on life span and free radical oxidation in Drosophila melanogaster. Mech. Ageing Dev., 1997, 97: 81-91.