Worm Breeder's Gazette 15(2): 56 (February 1, 1998)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.


Vladimir V. Baaev

box 45, Novosibirsk, 630107, USSR

The purpose of this study was to investigate the effect of different
concentrations of sodium adenosinetriphosphate in water solutions on
nematode life span. In this experiment sodium adenosinetriphosphate
was used in following dilutions : 1:10*3, 1:10*4, 1:10*5, 1:10*6 and
1:10*7. Three adult animals (3 - 5 days old) were kept in microtitre
wells containing 0.75 ml of liquid medium (with E. coli and without
sodium adenosinetriphosphate) during 4 hours, then they were discarded
and newborn larvae were transferred in next wells (with sodium
adenosinetriphosphate in any concentration) every day (one worm in one
well). This investigation was carried out in temperature +21C and in
the darkness. The obtained results are presented in the following

Concentration of                        Longevity (days)
adenosinetriphosphate     n        mean +/- S.E.       maximal

Control                  12        20.8 +/- 1.7          27

1:10*3                   12         5.6 +/- 0.2           6

1:10*4                   12         6.0 +/- 0.2           8

1:10*5                   12        10.7 +/- 1.2          20

1:10*6                   12        20.7 +/- 1.3          27

1:10*7                   12        20.9 +/- 1.8          26

The nematodes in concentration of sodium adenosinetriphosphate 1:10*3
were unable to have progeny, then, the concentrations 1:10*3 and
1:10*4 were toxic. The concentration 1:10*5 was slightly toxic (the
differences between longevities in concentrations 1:10*3, 1:10*4,
1:10*5 and in control were statistically significant, P < 0.001 in all
these cases). The longevities in concentrations 1:10*6 and 1:10*7 and
in control were similar (the differences between longevities in
concentrations 1:10*6 and 1:10*7 and in control were statistically not
significant, P > 0.05 in all these cases).

Conclusion: If the water solution of sodium adenosinetriphosphate was
applied to C. elegans during the whole life span in above described
conditions, it was not able to prolong the life span of these animals.

Acknowledgment: The author wishes to express his thanks to CGC for
providing C. elegans (Bristol, N2) and E. coli OP50.