Worm Breeder's Gazette 15(2): 56 (February 1, 1998)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
box 45, Novosibirsk, 630107, USSR
The purpose of this study was to investigate the effect of different concentrations of sodium adenosinetriphosphate in water solutions on nematode life span. In this experiment sodium adenosinetriphosphate was used in following dilutions : 1:10*3, 1:10*4, 1:10*5, 1:10*6 and 1:10*7. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.75 ml of liquid medium (with E. coli and without sodium adenosinetriphosphate) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with sodium adenosinetriphosphate in any concentration) every day (one worm in one well). This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Concentration of Longevity (days) sodium adenosinetriphosphate n mean +/- S.E. maximal Control 12 20.8 +/- 1.7 27 1:10*3 12 5.6 +/- 0.2 6 1:10*4 12 6.0 +/- 0.2 8 1:10*5 12 10.7 +/- 1.2 20 1:10*6 12 20.7 +/- 1.3 27 1:10*7 12 20.9 +/- 1.8 26 The nematodes in concentration of sodium adenosinetriphosphate 1:10*3 were unable to have progeny, then, the concentrations 1:10*3 and 1:10*4 were toxic. The concentration 1:10*5 was slightly toxic (the differences between longevities in concentrations 1:10*3, 1:10*4, 1:10*5 and in control were statistically significant, P < 0.001 in all these cases). The longevities in concentrations 1:10*6 and 1:10*7 and in control were similar (the differences between longevities in concentrations 1:10*6 and 1:10*7 and in control were statistically not significant, P > 0.05 in all these cases). Conclusion: If the water solution of sodium adenosinetriphosphate was applied to C. elegans during the whole life span in above described conditions, it was not able to prolong the life span of these animals. Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.