Worm Breeder's Gazette 14(5): 27 (February 1, 1997)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Psst! Wanna buy a Clock?

Bernard Lakowski, Siegfried Hekimi

Dept. of Biology, McGill University, 1205 Dr. Penfield Ave. Montreal, Quebec, Canada, H3A 1B1

In a recent paper we reported work on the genetic analysis of life span
in Clock mutants and numerous double mutants ( Lakowski and Hekimi 
(1996). Science 272, 1010-1013 ).  This work has generated many strain 
requests, so we have sent the following strains to the CGC:  

CB4876 clk-1(e2519) III  
MQ130 clk-1(qm30) III    
MQ125 clk-2(qm37) III    
MQ131 clk-3(qm38) II     
MQ124 clk-3(qm38) II; clk-1(e2519) III 
MQ225 clk-3(qm38) II; clk-2(qm37) III   
MQ141 clk-1(e2519) clk-2(qm37)/dpy-17(e164) III
MQ223 clk-3(qm38) II; gro-1(e2400)/dpy-17(e164) III
MQ224 clk-3(qm38) II; clk-1(qm30)/dpy-17(e164) III
MQ524 gro-1(e2400) clk-2(qm37)/ dpy-17(e164) III
MQ513 daf-2(e1370) clk-1(e2519) III
MQ415 age-1(hx546) fer-15(b26) II; gro-1(e2400) III

Notes on using these strains
1) All Clock mutations are maternally and zygotically rescued even in
   the double mutant strains.  
2) Strains homozygous for clk-2(qm37) and gro-1(e2400) are not viable 
   at 25C.  They can be studied at 25C if they are allowed to complete
   embryogenesis at 20C or below and then shifted to 25C.  MQ130 clk-1
   (qm30) also has difficulty at 25C, segregating many dead eggs, but 
   can be maintained at this temperature.
3) MQ124 clk-3(qm38) II; clk-1(e2519) III is a strain which is just 
   barely viable and can not be maintained at 25C.  This strain 
   produces a lot of dead eggs and sporadically becomes sterile during
4) The strains MQ141, 223, 224 and 524 have LGIII Clock mutations 
   balanced over dpy-17(e164) because these Clock double mutant 
   combinations are not viable as homozygotes, dying out over a few 
   generations.  dpy-17 was chosen because it lies about 0.2 cM to the
   left of clk-1 and gro-1 and only about 1 cM to the left of clk-2.
   dpy-17 is also convenient because it has a strong phenotype as an 
   L1 larva.  Homozygous double mutants should be identifiable because 
   their progeny take so long to hatch and to develop.