Worm Breeder's Gazette 14(5): 27 (February 1, 1997)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
Dept. of Biology, McGill University, 1205 Dr. Penfield Ave. Montreal, Quebec, Canada, H3A 1B1
In a recent paper we reported work on the genetic analysis of life span in Clock mutants and numerous double mutants ( Lakowski and Hekimi (1996). Science 272, 1010-1013 ). This work has generated many strain requests, so we have sent the following strains to the CGC: CB4876 clk-1(e2519) III MQ130 clk-1(qm30) III MQ125 clk-2(qm37) III MQ131 clk-3(qm38) II MQ124 clk-3(qm38) II; clk-1(e2519) III MQ225 clk-3(qm38) II; clk-2(qm37) III MQ141 clk-1(e2519) clk-2(qm37)/dpy-17(e164) III MQ223 clk-3(qm38) II; gro-1(e2400)/dpy-17(e164) III MQ224 clk-3(qm38) II; clk-1(qm30)/dpy-17(e164) III MQ524 gro-1(e2400) clk-2(qm37)/ dpy-17(e164) III MQ513 daf-2(e1370) clk-1(e2519) III MQ415 age-1(hx546) fer-15(b26) II; gro-1(e2400) III Notes on using these strains 1) All Clock mutations are maternally and zygotically rescued even in the double mutant strains. 2) Strains homozygous for clk-2(qm37) and gro-1(e2400) are not viable at 25C. They can be studied at 25C if they are allowed to complete embryogenesis at 20C or below and then shifted to 25C. MQ130 clk-1 (qm30) also has difficulty at 25C, segregating many dead eggs, but can be maintained at this temperature. 3) MQ124 clk-3(qm38) II; clk-1(e2519) III is a strain which is just barely viable and can not be maintained at 25C. This strain produces a lot of dead eggs and sporadically becomes sterile during culture. 4) The strains MQ141, 223, 224 and 524 have LGIII Clock mutations balanced over dpy-17(e164) because these Clock double mutant combinations are not viable as homozygotes, dying out over a few generations. dpy-17 was chosen because it lies about 0.2 cM to the left of clk-1 and gro-1 and only about 1 cM to the left of clk-2. dpy-17 is also convenient because it has a strong phenotype as an L1 larva. Homozygous double mutants should be identifiable because their progeny take so long to hatch and to develop.