Worm Breeder's Gazette 14(5): 24 (February 1, 1997)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
1 | Institute of Molecular Biolgy and Biochemistry, Simon Fraser University, Burnaby, BC, Canada, V5A 1S6 |
2 | Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada, V6T 1Z3 |
Since our last report in WBG 14(2), we have expanded the cosmid transgenic library to include other chromosomes. Beyond the 147 cosmid trangenics we have constucted for chromosome III, we have now constructed transgenic strains for 61 cosmids from chromosome IV, 17 from chromosome X, 12 from chromosome I, 10 from chromosome II, and 6 from chromosome V. This brings the total number of cosmids available in transgenic strains to 253. The approximate location of these cosmids are shown as black bars on the figure below:
Our ongoing rescue attempts utilizing these cosmid transgenics have resulted in seven essential
genes being placed in the interval between dpy-17 and unc-36 on chromosome III. These results are as
follows:
Rescued Rescuing Gene Allele Cosmid -------------------------------------------------- let -713 s2470 C05D11 s2175 C05D11 let-716 s2457 C16A3 let-721 s2447 C05D11 let-725 s2454 C05D11 s2800 C05D11 let-756 s2613 C05D11 s2809 C05D11 let-767 s2464 C31H11 s2176 C31H11 let-774 s2615 C23G10 mel-32 s2518 C05D11 --------------------------------------------------
For more information regarding the cosmid C05D11, please refer to
"SuperCosmid Strikes Again" by Greg Vatcher et al. this issue of WBG.
A list of cosmids available as transgenic strains, as well as those which are presently under
construction, is available via the internet at http://darwin.mbb.sfu.ca/imbb/dbaillie/cosmid.html. Strains can be ordered by sending an e-mailed
request to djanke@darwin.mbb.sfu.ca.
This work is being done in collaboration with the C. elegans Genome Sequencing labs, and with
funding from the Canadian Genome Analysis and Technology Program. Lethal mutations were initially
positioned on the genetic map utilizing deficiencies generated for the Genetic Toolkit Project. This
Toolkit is being constructed with a grant from the NIH.