Worm Breeder's Gazette 14(5): 22 (February 1, 1997)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

New RNA in situ fixative

Brian Harfe1, Andrew Fire2

1 The Johns Hopkins University Biology Graduate Program, The Carnegie Institution of Washington
2 The Carnegie Institution of Washington

        Recently, we learned of a new, cheap fixative that works very
well on sea urchin embryos [S.T.F. Molecular Biology Grade, Streck
Laboratories (1-800-228-6090)]. We have begun to use this fix instead of
the formaldehyde fix commonly used in worm RNA in Situ's. We are
following the procedure of Seydoux and Fire, except for the substitution
of an overnight incubation at room temperature in S.T.F fixative instead
of the fixation step from the Seydoux procedure. 
        With a ceh-27  probe, we see a hybridization signal about three
times stronger using the Streck fix than with the standard formaldehyde
fix. We have found that the S.T.F. fix can be stored at room temperature
and reused (at least six times).   

General in situ procedure: G. Seydoux and A. Fire. Whole-Mount in Situ
Hybridization for the Detection of RNA in Caenorhabditis elegans
Embryos. In "Caenorhabditis elegans, Modern Biological Analysis of an
Organism" (edited by H. Epstein and D. Shakes) 1995.

We thank C. Kirchhamer and G. Seydoux.