Worm Breeder's Gazette 14(4): 31 (October 1, 1996)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Rescue of dpy-17

Anne M. Smardon, Eleanor M. Maine

Department of Biology Syracuse University Syracuse, New York 13244

        We have rescued dpy-17 with the cosmid F54D8.  In our lab we are
attempting to rescue sog-10  (suppressor of glp-1)  which lies
approximately 0.4 map units to the left of dpy-17 on LGIIII.  We have
obtained several transgenic lines containing cosmids that map in this
region from David Baillie's cosmid transgenic library.  In the course of
our work we noticed that with strain BC05123:  +/+; sEx271 III [F54D8 +
pCes1943{rol-6(su1006)dm}]  we were not able to create a sog-10 dpy-17;
sEx271 (Dpy Roller) strain.  We tested this strain for rescue of dpy-17
by creating the following strain:   dpy-17(e164) unc-32(e189); sEx271.
The phenotype of this strain is non-Dpy Unc Roller.  This strain
produces the following progeny:

          genotype                                phenotype
        dpy-17 unc-32                           Dpy-Unc

        dpy-17 unc-32; sEx271           non-Dpy Unc Rollers

        There are five transcription units on cosmid F54D8 that have
been identified by the Green and Hillier Genefinder program used by the
Genome Project.  F54D8.1 is a large transcription unit that encodes a
protein in which the C-terminus contains 245 amino acids that are 48%
identical to the 245 terminal amino acids of sqt-1  (a cuticle collagen
gene).  We believe this is the dpy-17 coding region.
See WBG Volume 14(#4) for protein alignment of F54D8.1 with sqt-1.