Worm Breeder's Gazette 14(4): 16 (October 1, 1996)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
1 | Division of Molecular Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands |
2 | The Sanger Centre, Hinxton Hall, Hinxton, Cambridge CB10 1RQ, United Kingdom |
In an effort to establish a high density Tc1 STS map as an extension of the mapping strategy developed by Williams et al. (1), we shotgun sequenced Tc1 insertion sites present in the high Tc1 copy number strains RW7000, CB4000 and KR1787 (2). To map these Tc1 insertion sites, flanking sequences were compared to the genomic sequence. In principle, all Tc1 insertion sites will fall in place when the complete genomic sequence of C. elegans becomes available. So far, we mapped 151 polymorphic Tc1 insertion sites in 40 Mbp of genomic sequence; a density of about one insertion in every 265 kb. Information on these mapped Tc1 insertion sites is available through ACeDB. These Tc1 insertions can be used as polymorphic markers, but can also be used for deletion mutatgenesis when a Tc1 insertion is located in or close to a gene of interest. (1) Williams, B. D. (1995) in Caenorhabditis elegans: Modern biological analysis of an organism, eds. Epstein, H. F. & Shakes, D. C. (Academic Press, San Diego), pp. 81-96. (2) Korswagen et al. (1995) Worm Breeder's Gazette 13(5): 90. Mapped polymorphic Tc1 insertion sites: (Indicated are approximate position on the genetic map, the cosmid the Tc1 is inserted in and the position of that Tc1 insertion in the cosmid sequence. Strain designations are A. RW7000, B. CB4000 and C. KR1787) Chromosome I pkP5319 0.00 C09D4 14218 A pkP5422 0.04 F55F8 15300 A pkP5242 1.25 T23B3 1135 A pkP5383 2.22 F27D4 683 A pkP550 2.26 D2005 17254 A pkP652 2.43 T28B8 6269 B pkP596 2.43 T28B8 20095 A pkP979 2.54 T01G9 3827 A pkP5459 2.80 K02B12 12868 A Chromosome II pkP5453 -4.60 W06A11 4604 A pkP5030 -4.53 F31D5 25916 A pkP5235 -4.53 F31D5 25984 A pkP682 -4.53 B0286 31305 B pkP632 -4.52 T12C9 9085 A pkP908 -4.52 T05C1 11339 A pkP5167 -3.32 R05F9 11496 B pkP617 -3.32 R05F9 12719 A pkP5017 -2.07 F07F6 25039 C pkP557 -1.40 C17C3 19067 A pkP727 -1.40 C17C3 27303 B pkP597 -0.84 B0034 10606 A pkP738 -0.50 F13H8 12107 C pkP5091 0.23 T02G5 14855 B pkP5162 0.29 C27H5 20352 B pkP416 0.30 E04F6 31057 A pkP5179 0.32 C30G12 9985 B pkP504 0.45 F35D2 6363 B pkP5391 0.48 F18A1 3056 A pkP5241 0.67 T05H10 19209 A pkP5009 0.79 F49E12 4748 C pkP5184 0.79 F14E5 17096 B pkP605 0.85 F46C5 9487 A pkP955 1.20 D2013 909 A pkP5187 1.25 C06C3 18716 B pkP746 1.41 T19H5 8754 C pkP5170 1.45 AH6 10404 B pkP515 1.48 F35H8 19105 C pkP5299 1.52 E02H1 1150 A pkP735 1.70 C08H9 11316 C pkP758 1.70 C08H9 11489 C pkP613 2.89 C09G5 23572 A pkP5144 3.21 B0491 8802 B pkP5260 3.36 C05D12 24325 A pkP645 3.53 B0334 34659 B pkP5190 3.73 F07A11 32699 B pkP683 3.82 C47D12 28460 B Chromosome III pkP417 -26.3 F42G9 1945 A pkP583 -4.84 C36A4 22793 A pkP753 -4.13 E03A3 31006 C pkP409 -4.04 C03C10 25246 A pkP406 -3.70 R07E5 3225 A pkP524 -3.47 T23F11 13007 C pkP689 -3.40 T04A8 12234 B pkP410 -2.56 C35D10 22392 A pkP644 -2.56 C35D10 23225 B pkP5174 -1.75 F48E8 30331 B pkP601 -0.92 C56G2 39105 A pkP402 -0.80 C06E8 22591 A pkP415 -0.75 R151 13226 A pkP403 -0.66 C07H6 54473 A pkP400 -0.52 ZK652 14661 A pkP405 -0.48 C29E4 39173 A pkP411 -0.27 R05D3 2352 C pkP729 2.31 R01H10 23395 B Chromosome IV pkP701 3.12 C01G5 19492 B pkP5024 3.38 B0496 17856 A pkP664 3.59 C26B2 24688 B pkP672 3.67 B0218 3876 B pkP512 3,92 C09G4 26221 C pkP5151 4.58 K07F5 37251 B pkP614 4.58 C47E12 24453 A pkP528 4.61 R09E10 13343 C pkP576 4.63 F13B12 13504 A pkP5392 4.73 R102 21118 A pkP756 5.53 K08E4 3577 C pkP5333 5.94 F08G5 10566 A pkP570 6.02 F53B2 3686 A Chromosome V pkP5306 -5.51 C52D10 37203 A pkP5223 -1.69 F08F3 16667 A pkP5475 -1.69 F08F3 16447 A pkP585 0.62 C33G8 28366 A pkP5401 0.62 C33G8 28416 A pkP5132 0.79 C37C3 17798 B pkP5056 0.84 F58G4 11837 A pkP520 1.88 F21C10 30979 C pkP5168 2.24 R07B5 4346 B pkP5309 2.33 T07C12 23165 A pkP5337 2.42 F57A8 21726 A pkP5385 2.42 F57A8 24331 A pkP710 2.45 C08B6 2290 B pkP5175 2.70 C35A5 6704 B pkP534 3.43 T11F9 36422 C pkP554 3.44 F38B7 12234 A pkP5181 3.85 F55C5 20402 B pkP5393 4.04 K01D12 22580 A pkP667 5.31 C56A3 6919 B pkP945 5.70 R10D12 4086 A Chromosome X pkP5165 -19.0 C02H7 5126 B pkP670 -14.3 F48B9 6937 B pkP706 -14.0 F47G3 15263 B pkP5020 -13.1 M02F4 17362 A pkP687 -13.1 M02F4 30478 B pkP5386 -12.7 ZK816 25592 A pkP744 -9.52 C01C4 7940 C pkP5335 -8.93 F47B7 2364 A pkP733 -7.46 F15A8 14142 C pkP538 -6.11 F43C9 7107 C pkP526 -5.92 M03F4 8930 C pkP754 -5.68 C42D8 4187 C pkP743 -5.02 F55D1 592 C pkP511 -3.99 F49E10 37400 C pkP943 -3.64 C09B8 19591 A pkP507 -2.87 C15B12 13 B pkP5058 -2.84 C25B8 13152 B pkP5382 -2.15 C16E9 17742 A pkP618 -1.97 K03A1 26302 A pkP532 -0.65 C18A11 4886 C pkP5116 -0.59 C47D2 11528 A pkP5285 -0.58 R09F10 23808 A pkP960 -0.02 K01A12 3152 A pkP5037 0.06 F12D9 20611 B pkP588 0.78 F47E1 13960 A pkP704 1.01 F18G5 13626 B pkP643 1.51 R07B1 21127 B pkP668 1.58 F19C6 10958 B pkP976 1.64 ZC373 9484 A pkP5331 1.78 F41E7 18069 A pkP503 1.93 ZC504 21481 B pkP720 2.03 F59F5 19347 B pkP5495 4.86 C35C5 38107 A pkP5028 8.57 R09A8 24734 A pkP757 9.55 C52G5 11870 C pkP514 10.6 F11C1 21686 C pkP980 11.7 K09A11 19736 A pkP649 11.8 R04D3 2050 B pkP5348 14.9 F48F7 28800 A pkP966 16.3 T24C2 24299 A pkP5154 18.7 C27C12 25078 B pkP5149 21.0 F47C8 15566 B pkP573 22.9 T27A8 13387 A pkP521 24.1 T21F2 16242 C pkP5302 24.1 C30G4 8866 A pkP651 24.1 C33E10 6646 B pkP834 24.1 C33E10 17338 A pkP933 24.3 F20B4 16665 A